乙脑灭活疫苗生产研究中常规采用小白鼠脑胶法滴定乙脑病毒，这种方法需要动物和动物房而且判定结果需要14天，我们试用BHK－21细胞蚀斑法满定动脑病毒，判定结果的时间由原来14天缩短为4天，结果证明蚀斑法可代替小鼠法相比，蚀斑法具有快速、经济、操作简单和重复性强等优点 JE inactivated vaccine production studies routine use of mouse brain gel method of titration of JE virus, this method requires animal and animal room and the results of the determination of the need 14 days, we try BHK-21 cell plaque method full of fixed brain virus, determine Results of the time from the original 14 days to 4 days, the results show that plaque can replace the mouse method compared to plaque spot method has the advantages of fast, economical, easy to operate and reproducible