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目的：证实缺血性脑损伤主要是以细胞凋亡的方式发生。方法：用ＴＵＮＥＬ法标记检测损伤的神经细胞，用原位杂交组织化学法检测ｂｃｌ－２ｍＲＮＡ，用免疫组织化学法检测Ｂａｘ蛋白。结果：手术组检测到ＴＵＮＥＬ标记的阳性神经细胞主要位于海马ＣＡ１区，渐进性增多，第３天达到高峰，可检测到凋亡小体和正在形成凋亡小体的神经细胞。ｂｃｌ－２主要在海马ＣＡ３区表达，Ｂａｘ则主要在ＣＡ１区表达，两者均于再灌注８ｈ出现，２４ｈ阳性信号达到高峰，７２ｈ明显下降。结论：细胞凋亡是大鼠全脑缺血后海马区神经元丢失的重要原因，大鼠全脑缺血再灌注后海马区ｂｃｌ－２和Ｂａｘ的区域性表达可能参与海马不同区域缺血耐受性差异的形成。
Objective: To confirm that ischemic brain injury occurs mainly in the manner of apoptosis. Methods: TUNEL method was used to detect the damaged neurons. Bcl-2 mRNA was detected by in situ hybridization and Bax protein was detected by immunohistochemistry. Results: TUNEL-positive neurons detected in the operation group were mainly located in the hippocampal CA1 region, and gradually increased. They reached the peak on the third day, and apoptotic bodies and apoptotic body-forming neurons were detected. Bcl-2 was mainly expressed in hippocampal CA3 region, while Bax was mainly expressed in CA1 region, both of which appeared at 8h after reperfusion. The positive signal of bcl-2 peaked at 72h and decreased significantly at 72h. CONCLUSION: Apoptosis is an important reason for loss of neurons in hippocampus after global cerebral ischemia in rats. The regional expression of bcl-2 and Bax in hippocampus after global cerebral ischemia / reperfusion may play an important role in ischemia tolerance in hippocampus The formation of sexual differences.