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目的:观察活血解毒方对糖尿病大鼠视网膜色素上皮衍生因子(PEDF)的影响。方法:采用链脲佐菌素(65 mg.kg-1)一次性ip SD大鼠建立糖尿病大鼠模型,随机分为模型组和活血解毒方高、低剂量组(15.8,7.7 g.kg-1)及导升明组(0.167 g.kg-1),另设正常对照组。ig给药,20周后处死动物。腹主动脉取血检测血清中PEDF;摘除眼球应用免疫组织化学观察PEDF蛋白的表达;取视网膜组织,采用荧光定量PCR法检测视网膜PEDFmRNA的表达。结果:糖尿病模型组大鼠血清中PEDF的含量(89.96±18.12)ng.L-1,比正常组降低(P<0.01),与模型组相比,活血解毒方各剂量组的含量均上升。糖尿病模型组大鼠视网膜LSAB法标记染色病理切片中PEDF表达降低,积分吸光度(IA)为(10.49±2.37),与正常组相比(41.75±10.25)有显著性差异(P<0.01),活血解毒方各剂量组与模型组相比显著升高(P<0.01)。与正常组相比,PEDFmRNA在糖尿病模型组的表达降低,而活血解毒方各剂量组的表达比模型组升高。结论:活血解毒方能够升高糖尿病大鼠视网膜PEDF蛋白和mRNA的表达,从而对糖尿病大鼠视网膜起保护作用。
Objective: To observe the effect of Huoxue Jiedu Fang on retinal pigment epithelium-derived factor (PEDF) in diabetic rats. Methods: Diabetic rats were induced by streptozotocin (65 mg.kg-1) once-a-day SD rats and randomly divided into model group and high-dose and low-dose Huoxue Jiedu decoction group (15.8mg · kg- 1) and Dijiazhuangming group (0.167 g.kg-1), and another normal control group. ig administration, animals were sacrificed 20 weeks later. Serum PEDF was detected by blood sampling in the abdominal aorta; PEDF protein expression was observed by immunohistochemistry in the extirpated eyes; Retinal tissue was taken and the expression of PEDF mRNA was detected by fluorescence quantitative PCR. Results: The content of PEDF in serum of diabetic model group (89.96 ± 18.12) ng.L-1 was lower than that of the normal group (P <0.01). Compared with the model group, the content of PEDF in each group increased. Compared with the normal group, the level of PEDF expression in the diabetic retinopathy model group was lower than that in the normal control group (P <0.01) Each dose group of Jiedu Fang was significantly higher than that of model group (P <0.01). Compared with the normal group, the expression of PEDFmRNA in diabetic model group decreased, while the expression of each group of Huoxue Jiedu Fang increased than the model group. Conclusion: Huoxue Jiedu Fang can increase the expression of PEDF protein and mRNA in the retina of diabetic rats and thus protect the retina of diabetic rats.