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根据Ⅰ类和Ⅱ类SLG基因两端序列合成了特异引物 ,对西南农业大学和HRI (HorticultureResearchInterna tional,Wellesbourne)提供的不同亲和指数的甘蓝 (BrassicaoleraceaL .)材料进行了PCR分析。结果表明 :具Ⅰ类SLG基因的材料是强自交不亲和系 ;具Ⅱ类SLG基因的材料既包括强自交不亲和系 ,也包括弱自交不亲和系。因此 ,Ⅱ类SLG基因的存在可作为区别甘蓝自交亲和系和自交不亲和系的分子标记。进一步用 6种识别 4bp的限制酶对Ⅱ类SLG基因DNA片段进行了RFLP分析 ,HRI的自交不亲和材料和西南农业大学的弱自交不亲和材料具有明显的RFLP多态性 ,被进一步用于鉴定甘蓝弱自交不亲和系内的各S等位基因系。这为利用HRI材料对现有种质进行改良提供了分子依据。
Specific primers were synthesized based on the sequences of the two kinds of SLG genes of class I and class II. PCR analysis was performed on the Brassica oleracea L. materials with different affinity indices provided by Southwest Agricultural University and HRI (Horticulture Research Internationl, Wellesbourne). The results showed that the material of class I SLG gene was strongly self-incompatible and the material of class II SLG included both strong self-incompatible and weak self-incompatible. Therefore, the presence of the class II SLG gene can be used as a marker to distinguish between selfing and selfing incompatibility lines in cabbage. Furthermore, RFLP analysis of DNA fragment of SLG gene of type II was carried out by using 6 kinds of restriction enzymes which recognize 4bp. The self-incompatible material of HRI and weak self-incompatible material of Southwest Agricultural University had obvious RFLP polymorphism It was further used to identify each S allele within the weak selfing incompatibility line of Brassica. This provided a molecular basis for the improvement of existing germplasm using HRI materials.