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目的:探讨M-FISH技术在检测复杂核型中的价值。方法:联合应用常规细胞遗传学技术和M-FISH检测2例伴有复杂核型异常的急性髓细胞白血病患者。结果:常规细胞遗传学技术检测2例急性髓细胞白血病患者的核型分别是:病例1为46,xy,der(8)t(8;21),+mar;病例2为46,xx,r(1)(p36p11),del(5)(q22q34)der(8)t(8;21),+mar。而应用M-FISH检测2例中的标记染色体分别是:t(8;21;8)和t(21;8;18;1)。例1经过1次诱导缓解治疗未获得完全缓解,并很快死亡。例2在4次不同方案的诱导缓解治疗后才获得完全缓解。结论:M-FISH是一种有效的检测复杂核型异常的方法。伴有复杂变异的t(8;21)核型的AML患者预后似乎不良。
Objective: To investigate the value of M-FISH in detecting complex karyotypes. Methods: Two cases of acute myeloid leukemia with complicated karyotype abnormality were detected by routine cytogenetics and M-FISH. Results: The karyotypes of two cases of acute myeloid leukemia detected by routine cytogenetics were: case 1 was 46, xy, der (8) t (8; 21), + mar, case 2 was 46, xx, r (1) (p36p11), del (5) (q22q34) der (8) t (8; 21), + mar. The marker chromosomes detected by M-FISH in 2 cases were t (8; 21; 8) and t (21; 8; 18; 1) respectively. Example 1 After 1 induction of remission did not achieve complete remission, and died soon. Case 2 achieved complete remission after 4 different induction remission treatments. Conclusion: M-FISH is an effective method to detect complex karyotype abnormalities. AML patients with a complex variant of t (8; 21) karyotype appear to have poor prognosis.