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目的探讨VEGF对HaCaT细胞诱生型一氧化氮合酶蛋白表达的影响。方法采用MTT法进行细胞增殖实验;Westernblot检测iNOS蛋白表达;Griess法检测NO的含量。结果 VEGF能促进HaCaT细胞增殖;VEGF作用后HaCaT细胞iNOS蛋白的表达上调,HaCaT细胞上清中NO含量增加;加入L-NAME后,VEGF促进HaCaT细胞增殖作用降低。结论 VEGF能促进HaCaT细胞诱生型一氧化氮合酶表达,且后者与HaCaT细胞增殖相关。
Objective To investigate the effect of VEGF on the expression of inducible nitric oxide synthase in HaCaT cells. Methods Cell proliferation assay was performed by MTT method. The expression of iNOS protein was detected by Western blot. The content of NO was detected by Griess method. Results VEGF promoted the proliferation of HaCaT cells; the expression of iNOS protein was increased in HaCaT cells after treated with VEGF, and the content of NO in HaCaT cells was increased; the proliferation of HaCaT cells was decreased by L-NAME. Conclusion VEGF can promote the expression of inducible nitric oxide synthase in HaCaT cells, and the latter is related to the proliferation of HaCaT cells.