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Objectives: To investigate the expression of HPV16 mRNA innormal human keratinocytes transfected with pSV2-neo/16.Methods: First human keratinocytes were cultured in theserum-free medium M154.Second, the plasmid pSV2-neo/16was transfected into the human keratinocytes using atransfecting reagent. Third, RT-PCR and Southe Blottingwere used to detect the expression of HPV16 mRNA and DNAin the transfected keratinocytes, respectively.Results: The expression of HPV 16 mRNA was successfullyamplified and an 110bp was detected by RT-PCR. A 7.9kbfragment was confirmed in the transfected keratinocytes bySouthe Blot analysis.Conclusion: HPV 16 mRNA and DNA were successfullydetected in the human keratinocytes.