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目的:建立阿拉坦五味丸中没食子酸的含量测定方法。方法:色谱柱:Zorbax Eclipse XDB-C_(18)(150mm×4.6mm,5μm);流动相:甲醇-0.4%磷酸溶液(15:85);检测波长:273nm;柱温:35℃。结果:没食子酸进样浓度在0.0032～0.0961mg·ml~(-1)范围内线性关系良好(r=0.999 7),样品平均回收率为99.00%,RSD=0.87%。结论:此方法简便、准确,可用于阿拉坦五味丸中没食子酸的含量控制。
Objective: To establish a method for determining the content of gallic acid in Alatan Wuwei Pills. Method: Column: Zorbax Eclipse XDB-C_(18) (150mm×4.6mm, 5μm); mobile phase: methanol-0.4% phosphoric acid solution (15:85); detection wavelength: 273nm; column temperature: 35°C. RESULTS: The linear relationship was good in the range of 0.0032-0.0961 mg·ml-1 with gallic acid (r=0.999 7). The average recovery of the sample was 99.00%, and the RSD was 0.87%. Conclusion: This method is simple, accurate and can be used to control the content of gallic acid in Alatan Wuwei Pills.