1986年12月至1988年9月,我们从南京5所医院收集不明原因的腹泻病人粪便,进行隐孢子虫卵囊检查。每份粪便直接涂片后,先以金胺-酚染色法染色,-荧光镜过筛检查,发现阳性或可疑标本,再用改良抗酸染色法和沙黄-美蓝染色法染色,光学镜观察鉴别。被确认的阳性标本,继之以透射电镜观察,卵囊结构 From December 1986 to September 1988, we collected fecal samples of patients with unexplained diarrhea from five hospitals in Nanjing and examined the Cryptosporidium oocysts. After each stool was directly smeared, the cells were stained with Auramine-Phenol staining, screened by fluoroscope, positive or suspicious specimens were found, and then stained with modified acid-fast staining method and Shahuang-Methylene blue staining, and observed with optical microscope Identification. Confirmed positive specimens, followed by transmission electron microscopy, oocyst structure