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氧化铁颗粒标志干细胞是决定磁共振(MRI)能否成功观察干细胞活动的关键,主要的标志方法包括利用各种安全手段使干细胞更多摄取外源性氧化铁颗粒和利用转基因方法使干细胞内产生内源性氧化铁颗粒。然而,即使成功标志干细胞,实际工作中干细胞的MRI示踪成像仍面临干细胞分裂导致标志浓度下降、标志物向宿主细胞转移、干细胞如何定量分析、MRI各种参数如何平衡、如何从复杂的本底信号探明少量干细胞等诸多挑战,需进一步研究探索。
Iron oxide particle-labeled stem cells are the key to determine whether magnetic resonance (MRI) can successfully observe the activity of stem cells. The main markers include the use of various safety measures to make stem cells more exogenous iron oxide particles and genetically-generated stem cells Endogenous iron oxide particles. However, even with successful identification of stem cells, MRI tracer imaging of stem cells in actual work still faces the problem of stem cell division resulting in decreased marker concentration, transfer of markers to host cells, quantitative analysis of stem cells, how MRI parameters are balanced, and how to proceed from a complex background Signal to identify a small number of stem cells and many other challenges need further study and exploration.