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目的:建立了分子印迹固相萃取-高效液相色谱法检测食品中2种三唑类杀菌剂残留的方法。方法:分别以三唑醇、烯唑醇为模板分子,α-甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,采用本体聚合法合成分子印迹聚合物。将制得的三唑醇、烯唑醇聚合物按质量比1:1混合,制成固相萃取柱用于样品的前处理,并采用高效液相色谱法检测。结果:在低和高浓度添加水平下,平均回收率在78.7%～94.3%之间,RSD在1.8%～2.4%之间(n=5)。结论:该方法灵敏度高,精密度好,适合于同时检测食品中三唑醇和烯唑醇2种三唑类杀菌剂残留。
OBJECTIVE: To establish a method for the determination of two triazole fungicide residues in food by molecularly imprinted solid phase extraction - high performance liquid chromatography. Methods: The molecularly imprinted polymers were synthesized by bulk polymerization using triadimenol and diniconazole as template molecules, α-methacrylic acid as functional monomer and ethylene glycol dimethacrylate as crosslinking agent respectively. The obtained triadimenol and diniconazole polymer were mixed at a mass ratio of 1: 1 to prepare a solid-phase extraction column for pretreatment of the sample and detected by high-performance liquid chromatography. Results: The average recoveries were between 78.7% and 94.3% at low and high concentrations with RSD between 1.8% and 2.4% (n = 5). Conclusion: The method has high sensitivity and good precision and is suitable for the simultaneous determination of triadimenol and diniconazole triazole fungicides residues in food.