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AIM:To observe biological characteristics of hepatocar-cinoma cells before and after CD80 transfection and tocompare the effect of CD80-transfected hepatocarcinomacells on T lymphocyte activation.METHODS:Retro virus vector carrying CD80 genewas transfected into HepG2 cells to establish CD80-transfected hepatocarcinoma cells(HepG2/hCD80).Flowcytometry(FCM)was performed to detect CDS0 expres-sion in the transfected cells.RT-PCR was used to evalu-ate CD80 expression at mRNA level.In the presence ofanti-CD3 mAb,the proliferation of T lymphocyte wasobserved by MTT.Meanwhile,the expression of activatedmolecule marker CD25 was analyzed through FCM.RESULTS:A stable cell line HepG2/hCD80 expressingthe human CD80 was established.Growth curve showedthat the molecule CD80 could obviously decrease thegrowth of tumor cells.HepG2/hCD80 was evidenced tohave a potency to enhance T cell proliferation and up-regulate CD25 expression.CONCLUSION:CD80 transfection can lower ma-lignant phenotype of hepatocarcinoma cells.CD80transfection has a down-regulatory effect to activatedT cells in vitro.
AIM: To observe biological characteristics of hepatocar-cinoma cells before and after CD80 transfection and tocompare the effect of CD80-transfected hepatocarcinoma cells on T lymphocyte activation. METHODS: Retro virus vector carrying CD80 gene was transfected into HepG2 cells to establish CD80-transfected hepatocarcinoma cells ( HepG2 / hCD80) .Flowcytometry (FCM) was performed to detect CDS0 expres-sion in the transfected cells. RT-PCR was used to evaluate-ate CD80 expression at mRNA level. The presence of anti-CD3 mAb, the proliferation of T lymphocyte wasobserved by MTT.Meanwhile, the expression of activated molecule marker CD25 was analyzed through FCM.RESULTS: A stable cell line HepG2 / hCD80 expressing the human CD80 was established. Growth curve showed that the molecule CD80 could significantly reduce the growth of tumor cells. HepG2 / hCD80 was evidenced tohave a potency to enhance T cell proliferation and up-regulate CD25 expression. CONCLUSION: CD80 transfection can lower ma-lignant phenotype of hepatocar CD80 transfection has a down-regulatory effect to activated T cells in vitro.