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The paper explored the possibility of hairy root occurrence of peony induced by Agrobactrium rhizogenes.It showed that R1000 had the highest hairy root inducement with a hairy root rate of 35%,whereas strains A4,MSU-2 and MT232 had little difference in hairy root occurrence.Infect conditions had great influence on hairy root inducement,and the better ones are: acetosyringone concentration,30 mg·L-1;infection time,15 minutes;and culturing conditions of strains,temperature 28 ℃,28 hours shaking culture under dark environment with shaking.Pre-experiment on tissue culture system of peony was conducted,and got better medium composition as: WP+6-BA 0.5 mg·L-1+ IBA 0.1 mg·L-1+GA30.5 mg·L-1for base buds,and MS + 6-BA 2.0 mg·L-1+IBA 0.1 mg·L-1+GA3 0.5 mg·L-1for stem segments.
The paper explored the possibility of hairy root incidence of peony induced by Agrobactrium rhizogenes .It showed that R1000 had the highest hairy root inducement with a hairy root rate of 35%, while les A4, MSU-2 and MT232 had little difference in hairy root occurrence.Infect conditions had great influence on hairy root inducement, and the better ones are: acetosyringone concentration, 30 mg · L -1; infection time, 15 minutes; and culturing conditions of strains, temperature 28 ° C, 28 hours shaking culture under dark environment with shaking. Pre-experiment on tissue culture system of peony was conducted, and got better medium composition as: WP + 6-BA 0.5 mg · L -1 + IBA 0.1 mg · L -1 + GA30.5 mg · L- 1for base buds, and MS + 6-BA 2.0 mg · L-1 + IBA 0.1 mg · L-1 + GA3 0.5 mg · L-1for stem segments.