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采用PCR技术对广西钦洲湾水域的养殖牡蛎(传统上被认为是近江牡蛎CrassotreaariakensisFujita)群体26个个体的线粒体DNA16SrRNA基因片段序列进行扩增,获得了大约500bp的扩增产物。PCR产物经纯化后进行序列测定,经同源排序,得到415bp可供分析的核苷酸片段。26个个体中共检测到18个变异位点,包括1个碱基插入/缺失、11个转换位点及6个颠换位点。共有6种单倍型,这6种单倍型又分为2大类单倍型。运用MEGA软件计算出不同个体间的遗传距离,并构建了UPGMA和NJ系统树。26个个体聚成明显的2支,一支有19个个体,占73 08%;另一支有7个个体,占26 92%;2支间序列差异为3 54%。据此得出结论:钦洲湾养殖牡蛎应存在两大种群或是2个亚种,其差异是否到了种间的分界限,还需进一步研究证实。
The mitochondrial DNA 16S rRNA gene fragment was amplified by PCR from 26 individuals of the cultured oyster (traditionally known as Crassotreaaria kensis Fuji) in the Qinzhou Bay waters of Guangxi, and an amplification product of about 500 bp was obtained. After purification, the PCR products were sequenced and sequenced to obtain 415 bp nucleotide fragments for analysis. A total of 18 loci were detected in 26 individuals, including 1 base insertion / deletion, 11 loci and 6 loci. A total of six haplotypes, which six haplotypes are divided into two major haplotypes. Using MEGA software to calculate the genetic distance between different individuals, and constructed UPGMA and NJ phylogenetic tree. 26 individuals clustered into two distinct groups, one with 19 individuals, accounting for 73.08%; the other with 7 individuals, accounting for 26.92%; and the difference between the two sequences was 3 54%. Based on this, we concluded that there should be two major populations or two subspecies of cultured oysters in Qinzhou Bay. Whether the difference reaches the inter-species boundary limits needs to be confirmed by further studies.