快中子辐照对枯草芽孢杆菌DNA损伤研究

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采用脉冲场凝胶电泳技术检测并定量分析了CFBR-Ⅱ快中子脉冲堆产生的快中子在不同剂量和剂量率条件下,对枯草芽孢杆菌黑色变种(ATCC 9372)DNA双链断裂的诱导.通过DNA释放百分比PR值、DNA断裂水平L值、断裂DNA平均分子量和DNA片段分布等指标的分析,结果表明:在不同的辐射条件下,DNA片段均明显分布于两个区域,表明枯草芽孢杆菌黑色变种DNA分子上可能存在对中子辐射较为敏感的位点;并且随着中子辐射剂量和剂量率的变化,DNA释放百分比PR值、DNA断裂水平L值和各片段区双链断裂的含量也会发生一定规律性的变化.
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