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本文应用差值Fourier技术和制约立体化学参数最小二乘技术以二锌猪胰岛素模型为起点测定了1.9分辨率的(D-Ala)~(B0)猪胰岛素晶体结构,R因子为0.211,键长均方差为0.057。B_0残基的电子密度表现清晰。B_0的引入降低了B链N端肽段构象的运动性,使分子在晶体中的堆积更加紧密。(D-Ala)~(B0)猪胰岛素和二锌猪胰岛素结构的主要差异在于分子可能的受体结合表面中部分极性基团的构象和分子IA链两段螺旋的组装。(D-Ala)~(B0)猪胰岛素B链N端晶体学微环境与(Trp)~(B1)猪胰岛素和去B_1牛胰岛素有很大的差异。本文还简单地讨论了胰岛素分子结构与免疫功能的关系。
In this paper, D-Ala porcine insulin (D-Ala) ~ (B0) crystal structure with 1.9-point resolution was determined by using the differential Fourier technique and the least-squares technique to control the stereochemical parameters. The R factor was 0.211, The mean square deviation is 0.057. The electron density of the B_0 residue is clearly shown. The introduction of B 0 reduced the motility of the N-terminal peptide conformation in the B chain, resulting in a more compact packing of the molecules in the crystal. The main differences between the (D-Ala) ~ (B0) porcine insulin and dinadiazepin insulin structures are the conformation of the partial polar groups in the possible receptor-binding surface and the assembly of the two-stranded helix of the molecular IA chain. (D-Ala) ~ (B0) porcine insulin B chain N-terminal crystallographic microenvironment is very different from (Trp) ~ (B1) porcine insulin and to B_1 bovine insulin. This article also briefly discusses the relationship between insulin molecular structure and immune function.