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为进一步了解江苏省花生地方品种的遗传多样性,用25对SSR引物评价了133份地方品种,共扩增出93个等位基因,平均3.72个;多态性信息量(PIC)变幅为0.066~0.749,平均为0.374;Shannon’s信息指数变幅为0.161~1.385,平均为0.671。品种间相似系数变幅为0.416~1.000,平均为0.830,4种类型品种相似系数均值排序为:普通型(0.899)>龙生型(0.776)>珍珠豆型(0.710)>多粒型(0.700)。采用非加权平均法(UPGMA)进行聚类分析,在遗传相似系数0.600时,将133份地方品种划分为三大类群。研究结果表明,江苏省花生地方品种遗传多样性较丰富,普通型品种的遗传多样性低于其它3种类型。聚类分析结果为地方品种有效利用提供重要依据。
In order to further understand the genetic diversity of peanut landraces in Jiangsu Province, 133 landraces were evaluated with 25 pairs of SSR primers and a total of 93 alleles were amplified with an average of 3.72. The polymorphism information (PIC) 0.066 ~ 0.749, an average of 0.374; Shannon’s information index amplitude 0.161 ~ 1.385, an average of 0.671. The similarity coefficients of varieties ranged from 0.416 to 1.000 with an average of 0.830. The average values of the similarity coefficients of 4 cultivars were as follows: common (0.899)> wild (0.776)> pearl (0.710)> multiple (0.700) ). Cluster analysis was performed by using non-weighted average method (UPGMA). When the genetic similarity coefficient was 0.600, 133 landraces were divided into three groups. The results showed that the genetic diversity of peanut landraces in Jiangsu Province was rich, while the genetic diversity of common landraces was lower than the other three types. Cluster analysis results provide an important basis for the effective utilization of local varieties.