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长期以来,肿瘤转移灶检测方法仅限于裸眼和光学显微镜观察,灵敏度和准确度均不理想,是肿瘤转移研究方法学中的严重不足.电镜的分辨率显然高于裸眼与光学显微镜,但由于技术要求较高,应用一般制样方法进行电镜观察,不但方法繁琐而且影响因素很多.本实验应用甲基丙烯酸甲酯和丁酯二种单体的混合物灌注实验小鼠肺脏,聚合后形成铸型,用扫描电镜观察小鼠黑色素瘤B_(16)L_(10)肺转移灶并经DJS-051微型计算机行电镜图象伪彩色处理,建立了一种较现有方法更灵敏和精确的新技术,以检测实验性肿瘤早期肺转移灶.
For a long time, the detection method of tumor metastases was limited to naked eye and optical microscopy, and the sensitivity and accuracy were not ideal. It is a serious deficiency in the research method of tumor metastasis. The resolution of electron microscope is obviously higher than that of naked eye and optical microscope, but due to technology The requirements are high. Electron microscopy is used for general sample preparation methods. It is not only cumbersome but also has many influencing factors. In this experiment, a mixture of methyl methacrylate and butyl ester was used to perfuse the mice’s lungs and form a cast after polymerization. Scanning electron microscopy was used to observe the B_(16)L_(10) lung metastases of mice melanoma and DJS-051 micro-computers. The pseudo-color processing of TEM images was used to establish a new technique that is more sensitive and accurate than the existing methods. To detect early lung metastases in experimental tumors.