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目的探讨原人参二醇(Ppd)抑制肿瘤生长和促进肿瘤细胞凋亡作用及其机制。方法建立肝癌动物模型,将实验动物分为五组;对照组、阳性药组、Ppd低剂量组(Ppd 25 mg/kg)、Ppd中剂量组(Ppd 50 mg/kg)、Ppd高剂量组(Ppd 100 mg/kg),2 w后处死动物测定肿瘤的重量和体积并做组织切片进行原位末端标记染色(TUNEL)。结果Ppd给药组肿瘤生长被抑制,其肿瘤体积、重量均较对照组减小(P<0.01),TUNEL结果显示Ppd 100 mg/kg给药组细胞凋亡数量与对照组之间存在显著性差异(P<0.01)。结论Ppd具有抑制肿瘤细胞的增殖的作用,其作用机制的可能是促进肿瘤细胞的凋亡。
Objective To explore the mechanism and mechanism of protopanaxadiol (Ppd) in inhibiting tumor growth and promoting tumor cell apoptosis. Methods Animal models of liver cancer were established. The experimental animals were divided into five groups: control group, positive drug group, low-dose Ppd group (Pdd 25 mg/kg), middle-dose Ppd group (Pdd 50 mg/kg), high-dose Ppd group ( Ppd 100 mg/kg) was sacrificed after 2 weeks. The weight and volume of the tumor were measured and histological sections were stained for TUNEL. Results The tumor growth was inhibited in the Ppd group, and the tumor volume and weight were decreased compared with the control group (P<0.01). TUNEL results showed that the amount of apoptosis in the Ppd 100 mg/kg group was significant compared with the control group. Difference (P<0.01). Conclusion Ppd can inhibit the proliferation of tumor cells, and its mechanism may be to promote the apoptosis of tumor cells.