We have built an integrated imaging system by combining stimulated emission depletion(STED)microscope and atomic force microscope(AFM).The STED microscope was constructed based on the supercontinuum fiber laser and a super lateral resolution of42 nm was achieved.With this integrated imaging system,morphological features,mechanical parameters and fluorescence super resolution imaging were obtained simultaneously for both nanobeads and fixed cell samples.This new integrated imaging system is expected to obtain comprehensive information at the nanoscale for studies in nanobiology and nanomedicine. We have built an integrated imaging system by combining stimulated emission depletion (STED) microscope and atomic force microscope (AFM). The STED microscope was constructed based on the supercontinuum fiber laser and a super lateral resolution of 42 nm was achieved. Here this integrated imaging system , morphological features, mechanical parameters and fluorescence super resolution imaging were obtained for both nanobeads and fixed cell samples. this new integrated imaging system is expected to obtain comprehensive information at the nanoscale for studies in nanobiology and nanomedicine.