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目的探讨丹皮酚对人卵巢癌SKOV3细胞的促凋亡作用及其可能的机制。方法用25、50、100、200、400μg/ml丹皮酚处理SKOV3细胞,设未经丹皮酚处理的细胞为对照组,24 h后,采用MTT法检测细胞增殖情况;用50、100、200μg/ml丹皮酚处理SKOV3细胞,设未经丹皮酚处理的细胞为对照组,24 h后,采用流式细胞术(flow cytometry,FCM)、Hoechst染色法检测细胞凋亡情况,Western blot法检测caspase3及survivin蛋白的表达情况。结果与对照组相比,各浓度丹皮酚对SKOV3细胞的增殖均有明显的抑制作用,呈浓度依赖性(P<0.05),IC50值为200.06μg/ml;100、200μg/ml浓度组中SKOV3细胞凋亡率分别为(35.33±1.32)%、(39.56±1.27)%,与对照组[(9.01±1.21)%]相比明显增加(P<0.05);丹皮酚100、200μg/ml浓度组中发生凋亡的细胞数量较对照组多;丹皮酚处理后细胞凋亡相关蛋白survivin表达降低,而caspase3表达增加,高浓度组与低浓度组相比,差异均有统计学意义(P<0.05)。结论丹皮酚能显著抑制人卵巢癌SKOV3细胞增殖,促进细胞凋亡,其机制可能与其调控凋亡相关蛋白survivin、caspase3表达变化有关。
Objective To investigate the apoptosis-promoting effect of paeonol on human ovarian cancer cell line SKOV3 and its possible mechanism. Methods SKOV3 cells were treated with paeonol at 25, 50, 100, 200 and 400 μg / ml, cells without paeonol treatment were selected as control group. After 24 h, cell proliferation was detected by MTT assay. The cells treated with 200μg / ml paeonol were treated with paeonol, and the cells without paeonol were treated with paeonol for 24 hours. Flow cytometry (FCM) and Hoechst staining were used to detect the apoptosis of SKOV3 cells. Western blot Method to detect the expression of caspase3 and survivin protein. Results Compared with the control group, paeonol at various concentrations significantly inhibited the proliferation of SKOV3 cells in a concentration-dependent manner (P <0.05) with an IC50 value of 200.06 μg / ml. In the concentrations of 100 and 200 μg / ml (35.33 ± 1.32)%, (39.56 ± 1.27)%, respectively, which was significantly higher than that of the control group [(9.01 ± 1.21)%] (P <0.05) The number of apoptotic cells in the concentration group was higher than that in the control group. The expression of apoptosis-related protein survivin was decreased and the expression of caspase3 was increased after paeonol treatment. The differences were statistically significant between high-concentration group and low-concentration group P <0.05). Conclusion Paeonol can significantly inhibit the proliferation and promote the apoptosis of human ovarian cancer cell line SKOV3, which may be related to the regulation of the expression of survivin and caspase3.