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目的比较流式细胞术和鸡红细胞法检测小鼠腹腔巨噬细胞吞噬功能的一致性。方法取昆明种小鼠分为4组,某保健食品蛋白粉以0.13、1.33和4.00 g/kg.BW 3个剂量按0.3 ml/10 g.BW经口灌胃,另设阴性对照组给予去离子水。于30 d后用流式细胞术观察小鼠腹腔巨噬细胞吞噬荧光微球的能力。另取昆明小鼠40只,以同样方法分组和灌胃,用小鼠腹腔巨噬细胞吞噬鸡红细胞的方法进行比对。结果 2种方法中,中、低剂量组与对照组相比,吞噬百分率(percentage of phagocytosis,PP)及吞噬指数(phagocytosis index,PI)差异均无显著性(P>0.05);高剂量组两种方法 PP与对照组比差异均有极显著性(P<0.01),但PI有所不同,流式细胞术法与对照组比差异有极显著性(P<0.01),但鸡红细胞法差异有显著性(P<0.05)。结论应用流式细胞术检测小鼠腹腔巨噬细胞吞噬功能指标,与传统方法检测结果具有良好一致性。
Objective To compare the consistency of phagocytosis of murine peritoneal macrophages by flow cytometry and chicken erythrocyte assay. Methods Kunming mice were divided into four groups, a health food protein powder to 0.13,1.33 and 4.00 g / kg.BW three doses of 0.3 ml / 10 g.BW by oral gavage, another negative control group given to go Ionized water. After 30 days, the ability of mouse peritoneal macrophages to engulf fluorescent microspheres was observed by flow cytometry. Another 40 Kunming mice were grouped and gavage in the same way, and the mouse peritoneal macrophages swallowed chicken red blood cells for comparison. Results There was no significant difference in percentage of phagocytosis (PP) and phagocytosis index (PI) between the middle and low dose groups and the control group (P> 0.05) There was significant difference (P <0.01) between the methods of PP and the control group, but there was a difference in PI between the flow cytometry and the control group (P <0.01) There was significant (P <0.05). Conclusion The phagocytic function of mouse peritoneal macrophages was detected by flow cytometry, which was in good agreement with the traditional method.