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目的 探讨抑制α-catulin基因表达对胃癌细胞增殖、迁移和侵袭的影响,以及可能的机制.方法培养人胃癌细胞SGC-7901,根据转染物不同将细胞分为siRNA-α-catulin组、siRNA-对照序列组和空白对照组,MTT法检测细胞增殖能力,Transwell法检测细胞迁移和侵袭能力,Western blot检测细胞中α-catulin、N-钙粘蛋白(N-cadherin)、E-钙粘蛋白(E-cadherin)和波形蛋白(Vimentin)表达.结果siRNA-α-catulin组细胞24、48、72及96 h时吸光度A值均低于siRNA-对照序列组和空白对照组,差异有统计学意义(<0.05);与siRNA-对照序列组和空白对照组比较,siRNA-α-catulin组迁移细胞数和侵袭细胞数均降低,差异有统计学意义(<0.05);与siRNA-对照序列组和空白对照组比较,siRNA-α-catulin组细胞中α-catulin、N-cad-herin及Vimentin蛋白相对表达量均降低,而E-cadherin蛋白相对表达量增加,差异有统计学意义(<0.05).结论特异性沉默人胃癌细胞SGC-7901中基因表达可有效抑制细胞增殖、迁移和侵袭能力,其机制可能与抑制上皮-间质转化过程有关.“,”Objective To investigate the effect of genetic inhibition of α-catulin on proliferation, migration and invasion of gastric cancer cells. Methods Human gastric cancer cell line SGC-7901 were cultured and were divided into siRNA-α-catulin group, siRNA sequence control group and negative control group. MTT and Transwell assay were performed to measure cell proliferation and cell invasion, respectively. The levels ofα-catulin, N-cadherin, E-cadherin and Vimentin were determined by Western blot. Results MTT results indicated that optical density value at 24 h, 48 h, 72 h and 96 h in the siRNA-α-catulin group were significantly lower than that of siRNA-control sequence group and negative control group ( < 0.05). The amount of migrating cells and invasive cells in siRNA-α-catulin group were decreased dramatically compared with siRNA-control sequence group and blank control group ( <0.05). Expression levels of α-catulin, N-cadherin and Vimentin in siRNA-α-catulin group decreased while E-cadherin increased significantly when compared with siRNA-control sequence group and blank control group ( < 0.05). Conclusion Genetic knockdown of α-catulin gene in SGC-7901 cell line could prevent cell proliferation, migration and invasion by inhibition of transition of epithelial-mesenchymal.