目的:观察c-FLIP-L对TRAIL诱导乳腺癌MDA-MB-231细胞凋亡的影响及其具体机制。方法:构建靶向c-FLIP-L的siRNA质粒,转染乳腺癌MDA-MB-231细胞,RT-PCR、Weston blotting验证基因抑制效果。实验分空白对照组、TRAIL组、c-FLIP-L siRNA组和c-FLIP-L siRNA+TRAIL组共4组,MTT法检测各组MDA-MB-231细胞的增殖情况,流式细胞术检测MDA-MB-231细胞凋亡率,Transwell实验检测MDA-MB-231细胞侵袭能力,RT-PCR、Western blotting检测MDA-MB-231细胞中c-FLIP-L、caspase-3、caspase-8、MMP-2、MMP-9的表达。结果:靶向c-FLIP-L的siRNA质粒转染至乳腺癌细胞株可有效抑制c-FLIP-L mRNA[(37.12±3.02)vs(183.21±8.31),(174.65±10.06);P<0.05]及其蛋白的水平。c-FLIP-L siRNA和TRAIL联合处理MDA-MB-231细胞,与两者单独处理相比,细胞增殖抑制率显著升高[72 h时,(75.51±2.01)%vs(33.75±1.60)%,(34.31±2.01)%;均P<0.05],凋亡率显著升高[72 h时,(76.30±4.11)%vs(38.95±2.14)%,(29.28±1.66)%;均P<0.05],对细胞侵袭能力的抑制也显著增强。c-FLIP-L siRNA和TRAIL联合处理后,与两者单独处理相比,乳腺癌细胞中casepase-3、casepase-8的表达显著增强,而MMP-2、MMP-9的表达明显减弱。结论:抑制c-FLIP-L表达能够增强MDA-MB-231细胞对TRAIL的敏感性,从而提高诱导肿瘤细胞凋亡的能力,其机制可能与caspase-3、caspase-8的表达上调和MMP-2、MMP-9的表达下调有关。 Objective: To investigate the effect of c-FLIP-L on TRAIL-induced apoptosis in breast cancer MDA-MB-231 cells and its mechanism. Methods: siRNA plasmid targeting c-FLIP-L was constructed and transfected into breast cancer cell line MDA-MB-231. The gene inhibitory effect was verified by RT-PCR and Weston blotting. Four groups were divided into blank control group, TRAIL group, c-FLIP-L siRNA group and c-FLIP-L siRNA + TRAIL group. The proliferation of MDA-MB-231 cells in each group was detected by MTT assay. Flow cytometry The apoptosis rate of MDA-MB-231 cells and the invasion ability of MDA-MB-231 cells were determined by Transwell assay. The expressions of c-FLIP-L, caspase-3 and caspase-8 were detected by RT- MMP-2, MMP-9 expression. Results: The siRNA plasmid targeting c-FLIP-L transfected into breast cancer cell lines could effectively inhibit the expression of c-FLIP-L mRNA (37.12 ± 3.02 vs 183.21 ± 8.31, 174.65 ± 10.06, P <0.05 ] And its protein levels. The inhibitory rate of cell proliferation was significantly increased in combination with c-FLIP-L siRNA and TRAIL (75.51 ± 2.01% vs 33.75 ± 1.60% at 72 h) (34.31 ± 2.01)%, all P <0.05], and the apoptosis rate was significantly higher at 72 h (76.30 ± 4.11) vs (38.95 ± 2.14)% and (29.28 ± 1.66)%, respectively ], Inhibition of cell invasive ability is also significantly enhanced. After combined treatment of c-FLIP-L siRNA and TRAIL, the expression of casepase-3 and casepase-8 in breast cancer cells was significantly increased, while the expression of MMP-2 and MMP-9 was significantly decreased compared with the two treatment alone. CONCLUSION: Inhibition of c-FLIP-L expression enhances the sensitivity of MDA-MB-231 cells to TRAIL and enhances the ability of inducing apoptosis of tumor cells. The mechanism may be related to upregulation of caspase-3 and caspase-8, 2, MMP-9 expression is down-regulated.