Objective To investigate effect of p27kip1 and cyclinE proteins on the genesis and progression of human pancreatic cancer. Methods The expression of p27kip1 and cyclinE in tumor tissue and adjacent tissue of 32 patients with pancreatic cancer were detected by SP immunohistochemical technique.Results p27kip1 protein positive expression rate in tumor tissue of pancreatic cancer was 56% , which was lower than that in adjacent pancreatic tissue (P< 0.05),p27kip1 protein positive expression correlated significantly with tumor cell differentiation and lymphy node metastasis(P< 0.05);cyclinE positive expression rate was 69% , which was higher than that in adjacent pancreatic tissue(P< 0.05), cyclinE positive expression also correlated significantly with tumor cell differentiation and lymphy node metastasis(P<0.05). Conclusions p27kip1 and cyclinE proteins may play an important role in genesis and progression of pancreatic cancer.