论文部分内容阅读
目的探讨纳米二氧化硅(nano-SiO_2)暴露对人支气管上皮细胞(16 HBE)的损伤作用及维生素E对其的保护作用。方法根据CCK-8细胞毒性结果,选取对照,微米级SiO_2(micro-SiO_2,20μg/ml)、纳米nano-SiO_2(5、10和20μg/ml)和维生素E(20μg/ml nano-SiO_2+50μmol/L维生素E)处理16 HBE细胞24 h,采用倒置显微镜观察细胞形态改变,Hochest 33342和膜联蛋白V-碘化丙啶(PI)双染法检测细胞凋亡,RNA酶A消化和PI染色来检测细胞周期分布。结果当nano-SiO_2浓度达10μg/ml时,细胞活力明显降低(P<0.05),随着nano-SiO_2浓度进一步增高,细胞活力呈剂量依赖性降低;细胞体积缩小,核固缩,细胞的折光性减弱。随着nano-SiO_2浓度的增加,凋亡率呈剂量依赖性升高;而维生素E组较20μg/ml nano-SiO_2组,细胞凋亡率有所降低;细胞周期出现轻微的G1期阻滞,维生素E可以改善这种阻滞现象。结论维生素E干预对由于nano-SiO_2引起的16 HBE的凋亡及细胞周期改变具有一定的保护作用。
Objective To investigate the damage of human bronchial epithelial cells (16 HBE) induced by nano-SiO 2 and the protective effect of vitamin E on it. Methods According to the cytotoxicity of CCK-8, the effects of micro-SiO 2 (20μg / ml), nano-SiO 2 (5,10 and 20μg / ml) and vitamin E / L vitamin E) for 16 h, the morphological changes of the cells were observed by inverted microscope, and the apoptosis, RNase A digestion and PI staining were detected by Hochest 33342 and Annexin V-PI staining. To detect cell cycle distribution. Results When the concentration of nano-SiO 2 was 10 μg / ml, the cell viability was significantly decreased (P <0.05). With the further increase of nano-SiO 2 concentration, the cell viability decreased in a dose-dependent manner. The cell volume was reduced, the nuclear condensation and cell refraction Weakened. With the increase of nano-SiO 2 concentration, the apoptosis rate increased in a dose-dependent manner. Compared with 20 μg / ml nano-SiO 2 group, the apoptosis rate of vitamin E group decreased slightly; cell cycle showed slight G1 arrest, Vitamin E can improve this block phenomenon. Conclusion Vitamin E has some protective effects on the apoptosis and cell cycle of 16 HBE induced by nano-SiO 2.