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The ZnO films with two-dimensional ordered macroporous structure were successfully fabricated through hydrothermal crystal growth of ZnO on the ZnO substrate covered with a mouolayer of polystyrene (PS) spheres as template.The precursor solution of hydrothermal crystal growth of ZnO were prepared by equitramine (HMT).The confinement effect of the PS spheres template on the growth of ZnO nanorods and the influence of sodium citrate on the crystal growth of ZnO had been studied.The film surface morphology and the preferential growth of ZnO crystal were investigated by scanning electron microscopy (SEM) and X-ray diffraction (XRD),respectively.Also,the photoluminescence spectrum of ZnO films had been measured,and the corresponding mechanism was discussed.