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本工作用外源性 cAMP 加氨茶碱对小鼠艾氏腹水癌细胞进行了多方面的研究,观察到外源性3′,5′-cAMP 不仅对小鼠艾氏腹水癌细胞增殖有显著的抑制作用。在接种后第9天,看到癌细胞增殖受到抑制的同时,大多数癌细胞胞质内的 cAMP 免疫荧光细胞化学反应增强,癌细胞内3′,5′-cAMP 磷酸二酯酶(PDE)活性受到抑制,此外,还观察到某些癌细胞表面膜上微绒毛消失或变短以及对植物凝集素的凝集反应减弱等现象,这些癌细胞的逆转表现的变化均与不同瘤龄的癌细胞有关,如经外源性 cAMP 处理后,cAMP-PDE 活性降低出现的最早,均在接种后5～7天时最为明显,cAMP 免疫荧光细胞化学反应是在接种后第9天最为显著,癌细胞表面膜上的微绒毛消失和变短的细胞比数明显增多,对植物凝集素的凝集反应减轻都是在接种后7～9天时最为显著,而 cAMP 对小鼠艾氏腹水癌细胞的增殖抑制作用是在接种后第9～11天最为明显。根据上述实验结果,本文讨论了外源性 cAMP 加氨茶碱对腹水癌细胞的抑制作用和癌细胞内cAMP 荧光反应,3′,5′-cAMP-PDE 活性以及癌细胞表面膜上微绒毛形态学变化的因果关系。
In this work, exogenous cAMP and aminophylline were used to study Ehrlich ascites carcinoma cells in mice. It was observed that exogenous 3′,5′-cAMP not only significantly increased the proliferation of Ehrlich ascites carcinoma cells in mice. The inhibitory effect. On the 9th day after inoculation, while the proliferation of cancer cells was observed to be inhibited, cAMP immunofluorescence cytochemical reaction in the cytoplasm of most cancer cells was enhanced, and 3′,5′-cAMP phosphodiesterase (PDE) in cancer cells was enhanced. The activity was inhibited. In addition, the disappearance or shortening of microvilli on the surface membranes of some cancer cells and the weakening of the agglutination reaction of lectin on the surface of some cancer cells were observed. The changes in the reversal performance of these cancer cells were similar to those of cancer cells of different tumor ages. For example, after the treatment with exogenous cAMP, the earliest occurrence of cAMP-PDE activity was most evident at 5 to 7 days after inoculation, and the cAMP immunofluorescence cytochemical reaction was most pronounced on the 9th day after inoculation. The number of disappeared and shortened microvilli on the membrane increased significantly, and the agglutination response to lectin was most marked at 7 to 9 days after inoculation, whereas cAMP inhibited the proliferation of Ehrlich ascites carcinoma cells. It is most obvious on the 9th to 11th days after inoculation. Based on the above experimental results, this paper discussed the inhibitory effect of exogenous cAMP plus aminophylline on ascites cancer cells and intracellular cAMP fluorescence response, 3′,5′-cAMP-PDE activity, and microvilli morphology on the surface of cancer cells. Learn the causal relationship of change.