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目的：探讨辐射诱发人支气管上皮细胞(BEP2D)转化过程中肺癌相关基因的表达。方法：用Cartesian PixSys5500 cDNA Microarray点样仪将60个肺癌相关基因以微阵列形式点布于醛基化的玻璃片上。提取α离子辐射前BEP2D细胞（原代）和α粒子辐射后20代、35代细胞总RNA，经长片段反转录和线性扩增标记成荧光探针后与微阵列中cDNA进行杂交。结果：原代细胞中检测到40个基因表达；20代检测到47个基因表达；35代检测到20个基因表达。所检测的基因中，抑癌基因的mRNA丰度在原代和20代后细胞中急剧下降；大多数癌基因的表达丰度在20代以后细胞中仅轻微下降；生长因子类基因大都在20代细胞表达。结论：在辐射诱发的人支气管上皮转化细胞中，抑癌基因的失活可能与细胞恶化有关；癌基因及生长因子类基因可能促进了细胞的转化。
Objective: To investigate the expression of lung cancer-associated genes during radiation-induced human bronchial epithelial cells (BEP2D) transformation. Methods: 60 lung cancer-related genes were spotted on aldehyde-based glass slides using a Cartesian PixSys 5500 cDNA Microarray spotter. The total RNA of 20th generation and 35th generation cells after radiation of alpha ion radiation BEP2D cells (primary) and alpha particles was extracted, and was labeled with fluorescent probes by long fragment reverse transcription and linear amplification, and then hybridized with cDNA in the microarray. Results: 40 genes were detected in primary cells; 47 genes were detected in 20 passages; 20 genes were detected in 35 passages. Among the genes detected, mRNA abundance of tumor suppressor genes decreased dramatically in both primary and post 20th generation cells; the abundance of most oncogenes was only slightly decreased in cells after the 20th generation; most growth factor genes were in 20 generations. Cell expression. CONCLUSIONS: In radiation-induced human bronchial epithelial transformed cells, the inactivation of tumor suppressor genes may be related to cell deterioration; oncogene and growth factor genes may promote cell transformation.