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以南方根结线虫的抗病亲本P2(0897-2-1-2-3-1-2-1)、感病亲本P1(9905-1-2-1-1-1-1-1)及F1(P1×P2),F2(F1自交后代),BC1P1(Fl×P1)和BC1P2(Fl×P2)为试材,以南方根结线虫二龄幼虫为虫源,进行苗期二龄幼虫人工接种鉴定和病土盘栽自然发病鉴定,研究番茄南方根结线虫病的遗传规律。结果表明,人工接种鉴定的植株在接种15 d左右,感病植株开始发病,接种45 d后感病植株已充分发病,表现为植株矮小,叶片变小、变黄,部分植株下部叶片黄枯,有的枯死;病土盘栽自然发病鉴定的植株较苗期接种鉴定的植株迟12 d左右发病,病症相似。卡方检测表明,F1、F2、BC1P1和BC1P2群体中抗病植株和感病植株分别符合1:0、3:1、1:1和1:0基因模型,符合孟德尔遗传规律。认为该试验所采用的抗性材料P2对番茄南方根结线虫的抗性是受一对显性基因控制的,为该材料的利用奠定基础,也为抗南方根结线虫番茄品种的选育提供参考。
The resistant parent P2 (0897-2-1-2-3-1-2-1), susceptible parent P1 (9905-1-2-1-1-1-1-1) and F1 (P1 × P2) and F2 (F1 selfed), BC1P1 (F1 × P1) and BC1P2 (F1 × P2) were used as experimental materials. The second instar larvae of root-knot nematode Artificial identification of inoculation and identification of the natural disease of Panzhihua, and studying the genetic rule of southern root-knot nematode disease in tomato. The results showed that the plants inoculated with artificial inoculation appeared about 15 days after inoculation, and the susceptible plants started to develop. After inoculation for 45 days, the susceptible plants were well-developed, showing short plants, smaller leaves and yellowing, yellow leaves of some plants, Some died; soil disease planted naturally identified plants more seedling than the plants identified as late onset of disease 12 days, the symptoms are similar. The results of chi-square test showed that the resistant plants and susceptible plants in F1, F2, BC1P1 and BC1P2 populations fit the 1: 0, 3: 1, 1: 1 and 1: 0 genetic models, respectively, which accords with Mendelian inheritance. It is considered that the resistance of the resistant material P2 used in this experiment to M. meliloti is controlled by a pair of dominant genes, which lays a foundation for the utilization of this material and also provides breeding for resistance to Meloidogyne incognita tomato reference.