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为了研究不同来源款冬花药材之间的质量差异,本研究采用NMR代谢组学技术同时结合HPLC含量测定,对21份不同来源款冬花药材进行化学比较。主成分分析结果显示,款冬花栽培品和野生品可明显区分,说明两者化学组成存在一定差异。然后通过有监督的偏最小二乘法判别分析(PLS-DA)确定两者的成分差异。结果显示,野生品中咖啡酸、绿原酸、芦丁、款冬酮、甲基丁酸-3,14-Z-去氢款冬素酯(EMDNT)、款冬巴耳二醇(bauer-7-ene-3β,16α-diol)、谷甾酮等次级代谢产物含量高于栽培品,这与传统经验认为的野生品质量较优一致。肝毒性吡咯里西啶生物碱肾形千里光碱(senkirkine)在不同来源款冬花药材中也存在较大差异,相关性分析结果显示,该毒性成分与3,5-二咖啡酰基奎尼酸、3,4-二咖啡酰基奎尼酸、4,5-二咖啡酰基奎尼酸、芦丁、山柰酚类似物等次级代谢物存在正相关,且其相关性具有统计学意义(P<0.05)。款冬花药材中毒性成分和活性成分的相关性值得进一步深入研究。
In order to study the quality differences between different types of coltsfoot flower, we used NMR metabonomics combined with HPLC to determine the chemical constituents of 21 colts. Principal component analysis showed that the coltsfoot flower cultivars and wild products can be clearly distinguished, indicating that there is a certain difference between the two chemical composition. The supervised least-squares discriminant analysis (PLS-DA) was then used to determine the compositional differences between the two. The results showed that caffeic acid, chlorogenic acid, rutin, forskolin, EMDNT, bauer-7-ene -3β, 16α-diol), and the contents of secondary metabolites such as sitosterone were higher than those of cultivated products, which is consistent with the traditional experience that the quality of wild products is superior. Hepatic toxicity pyrrolizidine alkaloid nephropathy senkirkine in different sources of coltsfoot there is a big difference, the correlation analysis showed that the toxic components and 3,5-dicavo-acylquinic acid, 3,4-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, rutin, kaempferol and other secondary metabolites were positively correlated, and the correlation was statistically significant (P < 0.05). Colletotrichum toxic components and the relevance of active ingredients worth further study.