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For searching out male sterility-related proteins (polypeptides) in rice (Oryza sativa L.), we examined the difference of panicle protein (polypeptides) between hybrid rice (Wujin2A/R168, Wujin5A/R988) and their parents (male-sterile line Wujin2A, Wujin5A, and restorer line R168, R988) at the formation stage of pollen mother cell by two-dimensional electrophoresis (2-DE). The results revealed that the 2-DE polypeptide maps were similar among these experimental materials. A small group of polypeptides were disappeared in 2-DE polypeptide maps of male-sterile line (Wujin2A, Wujin5A) by comparing to restorer line (R168, R988) and the first filial (F_() generation (Wujin2A/R168, Wujin5A/R988). The isoelectric points of these polypeptides were pI 5.8-6.5, molecular weight 42.7×10~3-66.2×10~3.
For searching out male sterility-related proteins (polypeptides) in rice (Oryza sativa L.), we examined the difference of panicle protein (polypeptides) between hybrid rice (Wujin 2A / R168, Wujin 5A / R988) and their parents Wujin 2A, Wujin 5A, and restorer line R168, R988) at the formation stage of pollen mother cell by two-dimensional electrophoresis (2-DE). The results revealed the the 2-DE polypeptide maps were similar among these experimental materials. A small group The polypeptides were disappeared in 2-DE polypeptide maps of male-sterile line (Wujin 2A, Wujin 5A) by comparing to restorer line (R168, R988) and the first filial (F_ (generation) (Wujin 2A / R168, Wujin 5A / R988) isoelectric points of these polypeptides were pI 5.8-6.5, molecular weight 42.7 × 10 ~ 3-66.2 × 10 ~ 3.