超声粉碎和高速离心获得Ｃ．ｊ－Ｓ１３１ｌｙｓａｔｅ，ＳＤＳ－ＰＡＧＥ显示位于１０－９２ＫＤ间２４条蛋白条带，薄层扫描发现４３ＫＤ蛋白是菌体最主要成份，其次是１５ＫＤ和１７ＫＤ蛋白。Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ发现Ｃ。ｊ－Ｓ１３１ｌｙｓａｔｅ中４３ＫＤ蛋白抗原性最强，也最早诱导相应抗体产生。用凝胶过滤和液相制备型等电聚焦分离获得了纯化的９２ＫＤ、４３ＫＤ、１５ＫＤ三种蛋白。本文结果为进一步研究空弯菌诱致自身免疫综合征的分子机理打下了基础。 Ultrasound pulverization and high-speed centrifugation C. j-S131lysate, SDS-PAGE showed that there are 24 protein bands located between 10-92KD, the 43KD protein was found to be the main component of mycelium by TLC scanning, followed by 15KD and 17KD protein. Western blotting found C. The 43KD protein of j-S131lysate has the strongest antigenicity and also induces the corresponding antibody production as early as possible. Purified 92KD, 43KD and 15KD proteins were obtained by gel filtration and liquid-phase preparative isoelectric focusing. The results of this study lay the foundation for the further study of the molecular mechanism of Aerobic bacteria induced autoimmune syndrome.