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选用4只3岁体质量(55±2)kg装有永久性瘤胃瘘管的阉公羊,采用4×4拉丁方设计,研究纳米铜(日粮中添加量分别为0、10、20和30 mg/kg)对瘤胃液氨态氮(NH3-N)质量浓度及蛋白质质量浓度的影响。结果表明:纳米铜对瘤胃液NH3-N质量浓度影响不显著(P>0.05);添加10 mg/kg时绵羊瘤胃液微生物蛋白质平均质量浓度显著高于添加30 mg/kg(P<0.05),其他各组差异均不显著(P>0.05)。根据试验结果推断,纳米铜添加量低于30 mg/kg时不影响绵羊瘤胃液NH3-N质量浓度及微生物蛋白质质量浓度,而纳米铜的最适添加量为10 mg/kg。
Four 3-year-old castrated ram with permanent rumen fistula (55 ± 2 kg) were enrolled in a 4 × 4 Latin square design to study the effects of nano-copper (0, 10, 20, and 30 mg / kg) on ammonia nitrogen (NH3-N) concentration and protein concentration in rumen fluid. The results showed that nano-copper had no significant effect on the concentration of NH3-N in rumen fluid (P> 0.05). The average concentration of microbial protein in rumen fluid of sheep at 10 mg / kg was significantly higher than that of 30 mg / kg (P <0.05) Other groups showed no significant difference (P> 0.05). According to the experimental results, we conclude that when the content of nano-copper is less than 30 mg / kg, the concentration of NH3-N in the rumen fluid and microbial protein concentration in sheep are not affected, while the optimal dosage of nano-copper is 10 mg / kg.