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目的：探讨氧化苦参碱（ＯＭ）及甘草甜素（ＧＬ）对撤除苯巴比妥钠（ＰＢ）诱导的小鼠肝细胞凋亡的影响。方法：选用昆明种小鼠，测定肝／体质量比、肝组织ＤＮＡ含量，观察组织学改变及原位凋亡细胞ＴＵＮＥＬ标记等指标，观察腹腔内注射ＯＭ１５０ｍｇ／ｋｇ及ＧＬ５０ｍｇ／ｋｇ，３次／ｄ，于３６ｈ后，对撤除ＰＢ后引起的小鼠肝细胞凋亡的影响。结果：阳性对照组肝／体质量比及肝ＤＮＡ含量分别回落１６．３％及３２．４％；ＧＬ组分别回落１６．１％及２８．９％；ＯＭ组无明显回落。组织学检查及凋亡细胞ＴＵＮＥＬ标记显示：阳性对照组及ＧＬ组小鼠肝细胞出现典型凋亡改变，ＴＵＮＥＬ标记阳性；阴性对照组及ＯＭ组小鼠肝细胞未见明显凋亡改变。结论：ＯＭ可阻断撤除ＰＢ诱导的小鼠肝细胞凋亡，ＧＬ对其无影响。
Objective: To investigate the effects of oxymatrine (OM) and glycyrrhizin (GL) on hepatocyte apoptosis in mice induced by withdrawal of phenobarbital sodium (PB). METHODS: Kunming mice were used to measure liver/body mass ratio and liver tissue DNA content. Histological changes and in situ apoptotic cells TUNEL markers were used to observe intraperitoneal injection of OM150mg/kg and GL50mg/kg, 3 times/ d, After 36h, the effect of the removal of PB on the hepatocyte apoptosis in mice. RESULTS: The liver/body mass ratio and liver DNA content of the positive control group decreased by 16.3% and 32.4%, respectively. The GL group decreased by 16.1% and 28.9%, respectively. The OM group did not significantly decrease. Histological examination and apoptotic cell TUNEL markers showed that the positive control group and GL group showed typical apoptosis changes in hepatocytes and positive TUNEL markers. There was no obvious apoptosis in hepatocytes in the negative control group and OM group. CONCLUSION: OM can block the apoptosis of hepatocytes induced by PB-induced mice, but GL has no effect.