SMA基微孔膜固定化β-半乳糖苷酶的研究

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以超临界二氧化碳(SCCO2)为分散介质在聚偏氟乙烯(PVDF)微孔膜表面和孔内进行马来酸酐和苯乙烯的接枝共聚,合成出超高分子量的苯乙烯/马来酸酐交替共聚物(SMA)基微孔PVDF膜。以SMA基PVDF膜为载体通过酸酐基和酶分子上的氨基偶联,制备出具有酶催活性的功能性分离膜。考察了影响酶固定化的因素,确定其最佳固定化条件为:温度,4℃;pH,8.2;酶/膜,1:10;反应时间,6h。固定化酶膜的最适温度为55℃,最适pH为7.8,均比自由酶稍高;Km(0.3mmol/L)与自由酶接近。固定化酶膜活力达13
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