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目的：探讨ＨＬＡＡ０２型及其亚型与启东肝细胞癌的相关性。方法：用ＰＣＲＳＳＰ法分析启东肝细胞癌和正常人中ＨＬＡＡ０２型及其亚型的分布。结果：１０３例经病理诊断的肝细胞癌中５７例为ＨＬＡＡ０２型，其比例达到５５％，显著高于启东正常人群ＨＬＡＡ０２型３２％（３３／１０２）的比例，Ｐ＜０．００１。同时揭示包括ＨＬＡＡ０２型在内的ＨＬＡＡ位点的等位基因多为杂合型。启东肝细胞癌ＨＬＡＡ０２型中Ａ０２０１亚型所占的比例稍高于正常人群，但没有显著差异。分析比较表明，启东肝细胞癌中ＨＬＡＡ０２型的相对集中与当地肝癌中ｐ５３基因２４９密码子突变的高频度发生并没有关联。结论：在乙肝病毒和黄曲霉毒素均为危险因子的启东地区，ＨＬＡＡ０２型与肝细胞癌密切相关。
Objective: To investigate the relationship between HLA-A02 and its subtypes and Qidong hepatocellular carcinoma. METHODS: The distribution of HLA-A02 and its subtypes in Qidong hepatocellular carcinoma and normal controls was analyzed by PCR-SSP. Results: Among the 103 cases of hepatocellular carcinoma diagnosed by pathology, 57 cases were HLA-A02 type, the proportion of which reached 55%, which was significantly higher than the proportion of HLA-A02 type 32% (33/102) in Qidong normal population, P<0. 001. At the same time, it revealed that the alleles of HLA-A locus, including HLA-A02 type, were mostly heterozygous. The proportion of A0201 subtype in Qidong hepatocellular carcinoma HLA-A02 was slightly higher than that in the normal population, but there was no significant difference. Analysis and comparison showed that the relative concentration of HLA-A02 in the hepatocellular carcinoma of Qidong did not correlate with the occurrence of the high-frequency mutation of the 249 codon of the p53 gene in local liver cancer. Conclusion: In Qidong area where both hepatitis B virus and aflatoxin are risk factors, HLA-A02 is closely related to hepatocellular carcinoma.