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近几年,在分子生物学领域中,DNA分子遗传标记(DNAMoleuclar Gene-tic Marker)技术迅猛发展,新方法、新技术不断涌现。继同工酶(isozyme)、RFLP(Restriction FragmentLength Polymorphi-sm)和DNA指纹(DNA fingerprinting)技术以后,90年代又发展了RAPD(Random Amplifed Polymor-phic DNA)、CAPS(Cleaved Amplified Polymorphic Sequence)、AFLP(AmPlified Fragment Length Polymorphic)技术等,它们已越来越多地用于药材品种鉴别的研究。在已有的研究报道中使用的鉴别方法可归为三类:(1)基于PCR反应的方法,包
In recent years, in the field of molecular biology, DNA Molecular Marker (DNA) has rapidly developed, and new methods and technologies have emerged. Following the isozyme, RFLP (Restriction Fragment Length Polymorphi-sm) and DNA fingerprinting techniques, RAPD (Random Amplifed Polymor-phic DNA), CAPS (Cleaved Amplified Polymorphic Sequence), and AFLP were developed in the 1990s. (AmPlified Fragment Length Polymorphic) technology, etc., they have been increasingly used for the identification of medicinal species. The identification methods used in the existing research reports can be classified into three categories: (1) PCR-based methods, including