对原代人体嗜中性粒细胞的转染是很重要的,因为它实现了对人体发炎响应中通道的研究,而这在以前是很难实现的,原因在于培养嗜中性粒细胞很困难。通过这项研究,从细胞存活率及siRNA传递角度,研究者确立了最佳的嗜中性粒细胞转染的电穿孔条件。使用这些条件,可以进一步将该技术应用到包括干细胞在内的其他难以转染的原代细胞中。可以预见,这一领域将会在不久的将来蓬勃发展起来。 Transfection of primary human neutrophils is important because it enables the study of the pathways in the human inflammatory response that was previously difficult to achieve due to the difficulty of culturing neutrophils . Through this study, the authors established the optimal electroporation conditions for neutrophil transfection in terms of cell viability and siRNA delivery. Using these conditions, this technique can be further applied to other difficult-to-transfect primary cells, including stem cells. It is foreseeable that this area will flourish in the near future.