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目的探讨高氧致慢性肺疾病(CLD)早产鼠肺组织HoxB5基因表达规律及其对肺发育的影响。方法将80只早产鼠随机分为实验组及对照组,采用高浓度氧诱导早产鼠CLD模型,应用逆转录聚合酶链反应(RT-PCR)等技术,测定肺组织HoxB5 mRNA水平,并同时观察放射状肺泡计数(RAC)的变化。结果生后1、3d,两组HoxB5 mRNA水平及RAC差异无统计学意义(P>0.05),7d时两者均开始降低(P<0.05),14d继续下降,21d降至最低(P<0.01),且肺组织HoxB5 mRNA水平及RAC呈明显正相关(P<0.01)。结论暴露高氧中早产鼠肺组织HoxB5基因呈低表达状态,其表达水平与肺泡发育障碍程度相一致。
Objective To investigate the expression of HoxB5 gene in lung tissue and its effect on lung development in premature rats with hyperoxia-induced chronic lung disease (CLD). Methods Eighty premature rats were randomly divided into experimental group and control group. High concentration oxygen was used to induce CLD model in premature rats. Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the expression of HoxB5 mRNA in lung tissues. Radial alveolar count (RAC) changes. Results There was no significant difference in HoxB5 mRNA level and RAC between the two groups (P> 0.05) at 1 and 3 days after birth, both of them decreased at 7d (P <0.05), decreased at 14d and reached the lowest at 21d (P <0.01) ), And lung tissue HoxB5 mRNA level and RAC showed a significant positive correlation (P <0.01). Conclusions The HoxB5 gene in lung tissue of premature rats exposed to hyperoxia is in a low expression state, which is consistent with the degree of alveolar dysplasia.