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目的 观察APP770 转基因小鼠的空间学习记忆能力是否出现变化以及该变化是否伴随着胆碱乙酰基转移酶(ChAT)活性的改变。方法 选用鼠龄分别为 3个月、6个月和 9个月的转基因和非转基因雌性小鼠 ,每组为 8只。行为学研究的手段有Y迷宫、Morris型水迷宫和十字迷宫。行为学实验后 ,对小鼠脑组织不同部位 (皮层、海马、纹状体和下丘脑 )中胆碱乙酰基转移酶的活性进行测定。结果 与同龄对照小鼠比较 ,9个月的转基因小鼠空间学习记忆能力出现了明显的减退 ,并且其皮层和海马组织里ChAT的活性也明显减少 ,但纹状体和下丘脑里ChAT的活性则没有明显变化。鼠龄为 3个月和 6个月的转基因小鼠与对照鼠比较 ,其空间学习记忆能力则没有变化 ,并且ChAT的活性也基本没有改变。结论 9个月的转基因小鼠空间学习记忆能力出现了明显减退伴随着皮层和海马组织里ChAT活性的明显减少 ,提示转基因小鼠胆碱能系统的变化可能与学习记忆能力的改变有关。
Objective To observe whether there is any change in spatial learning and memory of APP770 transgenic mice and whether this change is accompanied by the change of choline acetyltransferase (ChAT) activity. Methods Transgenic and non-transgenic female mice of 3 months, 6 months and 9 months were selected, each group of 8 mice. Means of behavioral research include the Y maze, the Morris water maze and the maze. After behavioral experiments, the activity of choline acetyltransferase in different parts of mouse brain (cortex, hippocampus, striatum and hypothalamus) was determined. Results Compared with the control mice at the same age, the spatial learning and memory abilities of 9-month-old transgenic mice showed a significant decrease, and the activity of ChAT in cortex and hippocampus was also significantly reduced. However, the activity of ChAT in striatum and hypothalamus There is no significant change. Compared with the control mice, the transgenic mice at the age of 3 months and 6 months did not change their spatial learning and memory ability, and the activity of ChAT remained unchanged. Conclusions The spatial learning and memory abilities of 9-month-old transgenic mice showed a significant decrease accompanied by a significant decrease of ChAT activity in cortex and hippocampus, suggesting that changes of cholinergic system in transgenic mice may be related to changes in learning and memory ability.