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目的:探讨前列腺组织慢性缺血与前列腺增生(BPH)的关系。方法:选用雄性SD大鼠40只,随机分为实验组(用血管银夹套夹双侧髂总动脉建立大鼠前列腺缺血模型)和对照组(腹部切开分离髂总动脉,但不缩窄),每组各20只。术后饲养4周,称重,处死,取前列腺称重,并用ELISA法定量检测前列腺组织碱性成纤维细胞生长因子(bFGF)和缺氧诱导因子(HIF)-1α蛋白的表达。结果:两组术后死亡率及实验终点大鼠体质量无统计学意义。实验组前列腺湿重与指数均明显高于对照组(P<0.05)。实验组前列腺组织中bFGF和HIF-1α蛋白表达显著高于对照组(P<0.05),且bFGF蛋白浓度与前列腺湿重成一定线性相关(R2=0.91)。结论:慢性缺血是引起BPH的机制之一。
Objective: To investigate the relationship between chronic ischemia of prostatic tissue and benign prostatic hyperplasia (BPH). Methods: Forty male Sprague-Dawley rats were randomly divided into experimental group (model of prostatic hyperplasia by bilateral common iliac artery with vascular silver jacketed clip) and control group (incision of isolated common iliac artery without constriction Narrow), each group of 20. After 4 weeks of rearing, the rats were weighed, sacrificed and the prostate was weighed. The expression of basic fibroblast growth factor (bFGF) and hypoxia inducible factor (HIF) -1α in prostatic tissue were detected by ELISA. Results: There was no significant difference in body mass between the two groups in postoperative mortality and the end of experiment. The wet weight and index of prostate in experimental group were significantly higher than those in control group (P <0.05). The expression of bFGF and HIF-1α in the experimental group was significantly higher than that in the control group (P <0.05), and the bFGF protein concentration was linearly correlated with the wet weight of the prostate (R2 = 0.91). Conclusion: Chronic ischemia is one of the mechanisms that cause BPH.