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用乳鼠接种和组织培养方法从黄山市某医院婴儿室一次新生儿感染暴发流行的心血及粪便标本中分离到柯萨奇B3病毒，并用间接免疫荧光法从4名患儿和1名产妇的血清标本中查到抗柯萨奇B3病毒IgM抗体。实验证明本次暴发流行是由柯萨奇B3病毒感染引起的。
Coxsackie B3 virus was isolated from the blood and stool samples of a neonatal outbreak in the infant room of a hospital in Huangshan City using indirect inoculation and tissue culture methods. Four children and one maternal Anti-Coxsackie B3 virus IgM antibody was detected in serum samples. Experiments show that this outbreak is caused by Coxsackie B3 virus infection.