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目的:观察三氧化二砷(As2O3)瘤内给药对大鼠脑胶质瘤的疗效,以探讨胶质瘤的有效化疗手段。方法:实验用65只(180±10)g SD大鼠,5只做正常对照,在60只大鼠纹状体部埋植导管,其中30只通过导管接种9L胶质瘤细胞株,建立在体脑胶质瘤模型。15只注入等量D-Hanks平衡盐,作为阴性对照;另15只为安查组,进行药物安全性研究。于植入瘤细胞第9 d,30只模型鼠随机分为肿瘤组与治疗组(各15只)。治疗组及安查组通过导管将As2O3(10μmol/L)注入瘤内。阴性对照组和肿瘤组注等量生理盐水。(1)阴性对照组、肿瘤组、安查组和治疗组于注射As2O38 d后各随机处死5只,迅速剥离脑组织,测量肿瘤体积,HE染色,观察组织形态变化,免疫组化检测PCNA含量。(2)阴性对照组、肿瘤组、安查组和治疗组各剩余的10只大鼠,逐日称量体重,观察其行为学变化,待其自然死亡后计算生存时间,并取脑,验证肿瘤的存在。结果:治疗组肿瘤明显小于肿瘤组,大鼠体重下降较肿瘤组为轻。肿瘤组平均生存期为25.8天,治疗组平均生存时间40天。但治疗组有两只未计入平均,一只生存178天,另一只在研究结束时被处死(达14个月),且处死后未发现肿瘤。结论:As2O3可通过减缓脑胶质瘤的增殖,缓解大鼠体重下降,延长模型动物生存时间,对大鼠脑胶质瘤具有明显治疗作用。
Objective: To observe the efficacy of intratumoral administration of arsenic trioxide (As2O3) on glioma in rats and to explore the effective chemotherapy of glioma. METHODS: Sixty-five (180 ± 10) g SD rats were used as experimental controls and 5 normal controls. Catheters were implanted in 60 rat striatum, 30 of which were inoculated with 9L glioma cell lines through catheters. Brain glioma model. Fifteen mice were injected with the same amount of D-Hanks balanced salt as a negative control and the other 15 were Ancha group for drug safety study. On the 9th day after implantation of tumor cells, 30 mice were randomly divided into tumor group and treatment group (15 rats each). As2O3 (10μmol / L) was injected into the tumor via the catheter in the treatment group and the Ancha group. The negative control group and the tumor group were injected with normal saline. (1) In control group, tumor group, amnion group and treatment group, 5 rats were randomly sacrificed after As2O38d injection, and the brain tissue was rapidly dissected. The volume of tumor was measured. HE staining was used to observe the histological changes. The content of PCNA . (2) The remaining 10 rats in the negative control group, tumor group, Ancha group and treatment group were weighed daily to observe their behavioral changes. After the natural death, the survival time was calculated and the brain was taken to verify the tumor The presence. Results: The tumor in the treatment group was significantly smaller than that in the tumor group, and the weight loss in the rats was lighter than that in the tumor group. The average survival time was 25.8 days in tumor group and 40 days in treatment group. However, two of the treatment groups were not included in the mean, one surviving for 178 days, the other being sacrificed at the end of the study (up to 14 months) and no tumor was found after sacrifice. Conclusion: As2O3 can significantly reduce the glioma proliferation, alleviate the weight loss in rats and prolong the survival time of the model animals.