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用MS+5mg6BA+0.1mgIBA诱导辣椒子叶外植体分化出叶状体.用MS+3mg6BA+0.2mgIBA诱导出茎芽.再用MS+0.2mgIAA诱导生根,从而得到完整的小植株.直接以子叶外植体为受体,以Ti质粒为载体将CMVcp(黄瓜花叶病毒外壳蛋白)基因转化进辣椒,经再生性培养获得了转基因小植株.
The explants were induced to differentiate into phyllodes by inducing shoots with MS + 3mg6BA + 0.2mgIBA and rooting was induced with MS + 0.2mg IAA to obtain intact plantlets. Cotyledons The explant is a receptor, and the gene of CMVcp (cucumber mosaic virus coat protein) is transformed into pepper with Ti plasmid as vector, and transgenic plantlets are obtained through regenerative culture.