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以斑茅GXA87-36(♀)与割手密GXS79-9(♂)杂交获得的经形态鉴别为真杂种的后代材料GXAS07-6-1(斑茅割手密杂合体)及其双亲为材料,利用体细胞染色体计数以及SSR、SRAP分子标记对杂种进行真实性鉴定,并利用SRAP分子标记对斑茅GXA87-36、割手密GXS79-9及其杂种后代遗传位点的传递进行分析。结果表明,GXA87-36体细胞染色体数为2n=60,GXS79-9体细胞染色体数为2n=64,其杂种GXAS07-6-1染色体2n=62,其杂交的染色体遗传方式为n+n;筛选出3对SSR引物和5对SRAP引物可分辨后代的真伪;利用71对SRAP引物扩增后代和双亲,检测到1095个遗传位点,多态性位点数为800;母本有279个位点,其中有79%传递给其后代;父本有439个位点,其中有73%传递给后代;用NTSYS-pc2.10e软件计算Jaccard遗传相似系数,双亲间为0.474,后代与母本、父本间分别为0.696、0.752,表明后代GXAS07-6-1偏向父本遗传。
The hybrid progeny GXAS07-6-1 (Hermaphrodite hybrids) and its parents, which were obtained from the cross of the Pterocarya gativae GXA87-36 (♀) and GXS79-9 (♂) , The authenticity of hybrids was identified by somatic chromosome counts and SSR and SRAP molecular markers, and the SRAP molecular markers were used to analyze the transfer of the loci of the cotyledonary motifs GXA87-36, GXS79-9 and their hybrid progenies. The results showed that the chromosome number of GXA87-36 was 2n = 60, the chromosome number of GXS79-9 was 2n = 64, the chromosome 2n was 62 of the hybrid GXAS07-6-1, and the chromosome was inherited by n + n. Three pairs of SSR primers and five pairs of SRAP primers were screened to determine the authenticity of the offspring. Using 71 pairs of SRAP primers to amplify the progenies and their parents, 1095 genetic loci were detected with 800 polymorphic loci and 279 Of which 79% were passed on to their offspring. There were 439 paternal sites, of which 73% were passed on to offspring. Jaccard’s genetic similarity coefficient was calculated using NTSYS-pc2.10e software with a mean of 0.474 between parents and offspring , 0.696,0.752 between male parents, indicating that offspring GXAS07-6-1 bias paternal inheritance.