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目的:检测细胞周期蛋白E(Cyclin E)在人肺癌细胞系中的表达水平,探讨Cyclin E与人肺癌发生及其生物学行为的关系。方法:采用双重胸苷阻断法分别对人胚肺正常细胞系MRC-5和大细胞肺癌细胞系NCI-H460及小细胞肺癌细胞系NCI-H446进行同步化处理,流式细胞仪检测其同步化效果;应用蛋白质印迹法检测Cyclin E在上述3种同步化处理细胞中的表达水平。结果:Cyclin E蛋白在肺癌细胞系NCI-H460和NCI-H446中的表达水平明显高于人胚肺正常细胞系MRC-5,差异有统计学意义,P<0.01。同时比较两种肺癌细胞系,NCI-H460细胞的CyclinE蛋白的表达水平明显高于NCI-H446细胞,P<0.01。结论:CyclinE蛋白与肺癌的发生及生物学行为可能具有相关性。
OBJECTIVE: To detect the expression of Cyclin E in human lung cancer cell lines and to explore the relationship between Cyclin E and human lung carcinogenesis and its biological behavior. METHODS: Double-thymidine blockade was used to synchronize MRC-5 and NCI-H460 cell lines and NCI-H446 cell line respectively. Flow cytometry The effect of Cyclin E in the three kinds of synchronized cells was detected by Western blotting. Results: The expression of Cyclin E protein in lung cancer cell lines NCI-H460 and NCI-H446 was significantly higher than that in MRC-5 normal human embryo lung cells, the difference was statistically significant (P <0.01). At the same time, compared with the two lung cancer cell lines, the expression level of CyclinE protein in NCI-H460 cells was significantly higher than that in NCI-H446 cells (P <0.01). Conclusion: CyclinE protein may play an important role in carcinogenesis and biological behavior of lung cancer.