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目的探讨通心络对局灶性脑缺血再灌注损伤大鼠的保护作用。方法将60只SD大鼠随机分成假手术组、模型组、通心络低、中、高剂量组(0.3,0.6,1.2 g·kg?1),每组12只。线栓法制作大鼠脑缺血再灌注模型,于缺血30 min后再灌注,24 h后进行神经行为学评分,采用TTC法测量脑梗死体积,HE染色观察脑组织的病理改变,免疫组化法观察Fas蛋白的表达。结果与模型组比较,通心络低、中、高剂量组均能显著改善大鼠的神经系统损伤症状,缩小脑梗死灶,明显下调Fas蛋白的表达(P<0.01);并且通心络高剂量组与低剂量组比较,差异具有统计学意义(P<0.05)。结论通心络可通过抑制Fas蛋白的表达,减轻脑缺血后神经功能损伤,从而保护脑组织。
Objective To investigate the protective effect of Tongxinluo on focal cerebral ischemia-reperfusion injury in rats. Methods Sixty SD rats were randomly divided into sham operation group, model group and Tongxinluo low, medium and high dose groups (0.3, 0.6, 1.2 g · kg -1), with 12 rats in each group. The rat model of cerebral ischemia-reperfusion was established by thread occlusion. The rats were reperfused 30 minutes after ischemia and neurobehavioral scores were measured 24 hours later. The volume of cerebral infarction was measured by TTC method. The pathological changes of brain tissue were observed by HE staining. Fas method to observe the expression of the protein. Results Compared with model group, Tongxinluo low, medium and high dose groups could significantly improve the symptoms of nervous system injury, reduce cerebral infarction, and significantly down-regulate the expression of Fas protein (P <0.01); and Tongxinluo The difference between the dose group and the low dose group was statistically significant (P <0.05). Conclusion Tongxinluo can protect the brain tissue by inhibiting the expression of Fas protein and alleviating the neuronal damage after cerebral ischemia.