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AIM: To investigate the changes of lymphocyte sub-populations, especially CD4+CD25+ T regulatory cells in Smad3-/- mice. METHODS: Hematological changes and changes of lymphocyte subpopulations were detected in Smad3-/- mice using cell counter and flow cytometry, respectively, and compared to their littermate controls. RESULTS: The numbers of neutrophils and lymphocytes in peripheral blood were significantly increased in Smad3-/- mice compared to littermate controls. CD19+ expressing cells in blood and spleen, and CDS+ T cells in thymus were all markedly decreased in Smad3-/- mice. More important, Smad3-/- mice had an increased population of CD4+CD25+ T cells in peripheral lymphoid tissues, including thymus, spleen, and lymph nodes. CONCLUSION: These observations suggest that the changes of lymphocyte subpopulations might play a role in susceptibility to inflammation of Smad3-/- mice.
METHODS: He investigate the changes of lymphocyte subpopulations were detected in Smad3 - / - mice using cell counter and flow cytometry, respectively, and compared to their littermate controls. RESULTS: The numbers of neutrophils and lymphocytes in peripheral blood were significantly increased in Smad3 - / - mice compared to littermate controls. CD19 + expressing cells in blood and spleen, and CDS + T cells in thymus were all markedly decreased in Smad3 - / - mice. More important, Smad3 - / - mice had an increased population of CD4 + CD25 + T cells in peripheral lymphoid tissues, including thymus, spleen, and lymph nodes. CONCLUSION: These observations suggest that the changes of lymphocyte subpopulations might play a role in susceptibility to inflammation of Smad3 - / - mice.