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目的了解广州地区献血人群中艾滋病病毒1型(HIV-1)感染的流行病学情况。方法对广州地区581656例无偿献血者标本用2个不同厂家的ELISA法试剂同时检测抗-HIV,双试剂阳性者送广州市疾控中心用蛋白免疫印迹试验(WB)进行确认,从WB法阳性的献血者的血浆中提取核酸,用RT-PCR进行gag区基因扩增,对扩增产物进行测序和序列分析,并进行基因分型,用spss16.0软件对流行病学资料进行统计。结果 ELISA法抗-HIV双试剂阳性133例(0.023%,133/581 656),WB法检测120例HIV-1抗体阳性(0.021%,120/581 656),不确定13例,120例WB法阳性的献血者中有6种亚型:CRF01-AE重组亚型51例;CRF07-BC重组亚型32例;CRF08-BC重组亚型15例;01B型16例;B’型6例,通过异性性传播组的阳性率高于其他途径传播组,男性组高于女性组,外地身份证的献血者高于广州市身份证者,首次献血者高于再次献血者,文化程度为初中及以下者高于其他组,年龄26~35岁组高于其他年龄组。结论本地区献血人群中HIV-1感染者以外地男性青壮年为主,主要通过性传播。
Objective To understand the epidemiology of HIV-1 infection in blood donors in Guangzhou. Methods A total of 581,656 unpaid blood donors in Guangzhou were tested simultaneously by two different manufacturers of ELISA reagents for anti-HIV, and two reagents were sent to Guangzhou CDC for Western blotting. The blood samples were collected from blood donors. The gag region was amplified by RT-PCR. The amplified products were sequenced and sequenced. The genotypes were also obtained. The epidemiological data were analyzed by SPSS16.0 software. Results There were 133 positive cases (0.023%, 133/581 656) of anti-HIV dual-ELISA in ELISA method, 120 cases of HIV-1 antibody positive in WB method (0.021%, 120/581 656) There were 6 subtypes of positive blood donors: 51 cases of CRF01-AE recombinant subtype, 32 cases of CRF07-BC recombinant subtype, 15 cases of CRF08-BC recombinant subtype, 16 cases of 01B type and 6 cases of B ’ The positive rate of heterosexual transmission group was higher than that of other transmission groups, the male group was higher than the female group, the foreign ID card was higher than that of Guangzhou ID card, the first blood donors were higher than the second blood donors, the education level was junior high school and below Those in the age group of 26-35 years old were higher than the other age groups. Conclusion HIV-1 in the blood donors in this area are mostly young and middle-aged in other regions, mainly through sexual transmission.